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Near-Infrared Solutions for Multiplexed Confocal Imaging
FV3000 Red System

Interested in imaging with far-red fluorescent probes? Expand your multiplex capabilities with near-infrared (NIR) modules for the FV3000 microscope, including a 730 nm or 785 nm diode laser and GaAs PMTs for high-sensitivity detection up to 890 nm.

  • Greater multiplexing capabilities
  • Reduced autofluorescence
  • Gentler live-cell imaging
  • Deeper imaging with less absorption and scattering
FV3000 product image

Learn more about NIR and multiplexing

NIR Laser Diodes

  • Less cross-talk when selecting dyes
  • Laser diodes (LD730/785) designed to be driven in continuous wave (CW) mode, offering stability, low maintenance, and a long lifetime

LD730/785 for NIR Imaging

Applicable dyes for NIR imaging

Laser Fluorescent dye λ_Ex (nm) λ_Em (nm)
LD730 ATTO 725 727 752
Cy7 743 767
ATTO 740 743 763
DiR 748 780
DyLight 750 751 772
Alexa Fluor 750 752 779
LD785 IRDye 800RS 767 785
DyLight 800 770 795
IRDye 800CW 778 794
Alexa Fluor 790 782 805
Cy7.5 790 810

Quantum Efficiency

Cooled GaAs PMT Detector for NIR Imaging

  • Significantly extends the detection range in the near-infrared
  • High sensitivity up to 890 nm
  • One or two GaAs PMT detectors can be combined with GaAsP or standard PMT detectors, enabling up to six individual detectors for multiplex imaging from the VIS to NIR
  • Efficient cooling reduces thermal noise emitted from the photocathode for a high signal-to-noise ratio at extremely low light levels

High Transmittance Optics for NIR Imaging

High transmittance from VIS to IR with silver-coated scan mirrors and Olympus’ unique 1600 Coating on the scan lens.

Reflenctance of Scan Mirrors

Transmittance of Scan Lens

Deep Tissue Observation with Silicone Oil Immersion Objectives in the NIR

  • NIR fluorescence works well for deep imaging because NIR light scatters less in live tissue
  • The refractive index of silicone oil (ne≈1.40) is close to that of living tissue (ne≈1.38), enabling high-resolution observations deep inside living tissue with minimal spherical aberration
  • The UPLSAPO30XIR (NA 1.05, WD 800 μm) offers higher NIR transmittance thanks to Olympus’ 1600 Coating
     

A line objectives

UPLSAPO30XIR (NA 1.05, WD 800 μm) offers higher NIR transmittance

Multiplexing with X Line High-Performance Objectives

  • Greater multiplexing imaging is possible using additional NIR detection channels
  • Reduced chromatic aberration during co-localization analysis (corrected at 400 nm–1000 nm)
  • Higher numerical aperture, excellent image flatness, and high transmittance from UV to NIR

Chromatic Correction

X line objectives

TruFocus Red Z-Drift Compensator

  • Uses a near-infrared laser to locate the coverslip interface and corresponding focal position
  • Near-infrared light minimizes cell damage during prolonged observation
  • ​Works well for live cell imaging with far-red and near-infrared fluorophores

TRU FOCUS logo

On-Demand Webinar

Related Webinar

This webinar will focus on fluorescence multiplexing and deep tissue imaging using near infrared (NIR) laser light. NIR laser sources can help in visualizing biological structures more clearly and at higher resolution deep within the specimen. NIR excitation can also enable the use of more fluorescent dyes without spectral overlap.

Watch this webinar

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*Banner image: Rat brain slice labeled with Hoechst (blue), anti-IBA1 (Alexa Fluor 488; green), anti-MAP2 (Alexa Fluor 594, yellow), anti-FOX3/NeuN (Alexa Fluor 647; red), and anti-MBP (Alexa Fluor 750; cyan). Images were acquired using a UPLXAPO10X objective with 405 nm, 488 nm, 561 nm, and 730 nm laser lines on GaAsP and GaAs detectors. Maximum intensity projection in Z with TruSight deconvolution processing. Sample courtesy of EnCor Biotechnology.

*Product not available in all areas. Contact your local Olympus representative for more information.

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